Choosing the right illumination system for a light microscope can provide the best wavelength specificity, speed and contrast for each microscopy experiment, while reducing the phototoxicity that could damage a sample and skew results. However, the light source can easily be overlooked when considering how to maximise the performance of a widefield microscope.
In the latest issue of Biophotonics, Technical Content Specialist at CoolLED, Dr Isy Goodhand, explores the latest developments in LED Illumination technology. The article explains how this technology, when used alongside well-considered optical filters, can enhance the performance of widefield microscopy – and is also a simple and cost-effective approach.