Authors

Chen Zhang,1 Anmo J. Kim,2,3 Crisalesandra Rivera-Perez,4 Fernando G. Noriega,5,6 and Young-Joon Kimcorresponding author1

Affiliations

"1School of Life Sciences, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005 Republic of Korea
2Department of Electronic Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763 Republic of Korea
3Department of Artificial Intelligence, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763 Republic of Korea
4Department of Fisheries Ecology, Consejo Nacional de Ciencia y Tecnología, Centro de Investigaciones Biológicas del Noroeste, 23096 La Paz, Baja California Sur Mexico
5Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL USA
6Department of Parasitology, University of South Bohemia, České Budějovice, Czech Republic
Young-Joon Kim, Email: [email protected]
corresponding authorCorresponding author."

Topic

Neuroscience

Abstract

"Vitellogenesis (yolk accumulation) begins upon eclosion and continues through the process of sexual maturation. Upon reaching sexual maturity, vitellogenesis is placed on hold until it is induced again by mating. However, the mechanisms that gate vitellogenesis in response to developmental and reproductive signals remain unclear. Here, we have identified the neuropeptide allatostatin-C (AstC)-producing neurons that gate both the initiation of vitellogenesis that occurs post-eclosion and its re-initiation post-mating. During sexual maturation, the AstC neurons receive excitatory inputs from Sex Peptide Abdominal Ganglion (SAG) neurons. In mature virgin females, high sustained activity of SAG neurons shuts off vitellogenesis via continuous activation of the AstC neurons. Upon mating, however, Sex Peptide inhibits SAG neurons, leading to deactivation of the AstC neurons. As a result, this permits both JH biosynthesis and the progression of vitellogenesis in mated females. Our work has uncovered a central neural circuit that gates the progression of oogenesis.

Subject terms: Molecular neuroscience, Sexual behaviour, Entomology

DOI: https://dx.doi.org/10.1038/s41467-022-28592-2

Extract

Stimulation light pulses for CsChrimson-expressing cells were generated by a green channel of an LED illumination system (pE-300, CoolLED) and passed through two long-pass filters that have a 610 nm cut-off wavelength (Edmund Optics).

Product Associated Features

The pE-300white is a popular illuminator for everyday fluorescent screening and analysis with simple operatation and individual irradiance control of each LED channel.

Product Type

pE-300white

Journal

Nature Communications

Year of Publication

2022

Country of Publication

UK