Droplet microfluidic-based loop-mediated isothermal amplification (dLAMP) for simultaneous quantification of multiple targets

Authors

Ya-Ling Tan et al.

Topic

Molecular Biology

Extract

"Acquire fluorescence images of the entire microwells using an inverted confocal laser scanning microscope under 10× objective.
Preparing. Place the droplet counting microwell chip on the microscope counter. Open the imaging software and select appropriate scan modes.
Note: Nikon Inverted Eclipse Ti epifluorescence microscope equipped with a motorized XY stage (Nikon), a CoolLed pE-4000 illumination source, a Nikon DS-Qi2 camera, and an apochromatic 10× objective (numerical aperture, 0.45) (Nikon) is used here."

Product Type

pE-4000

Journal

Star Protocols

Year of Publication

2022

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