Slingenbergh, W., De Boer, S. K., Cordes, T., Browne, W. R., Feringa, B. L., Hoogenboom, J. P., … Van Dorp, W. F.


Applied Physics, Molecular Microscopy Research Group & Single-Molecule Biophysics, and Stratingh Institute for Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands and Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, 2628 CJ Delft, The Netherlands.

Application Area

Science Technology

fluorescence imaging


The controlled positioning of nanostructures with active molecular components is of importance throughout nanoscience and nanotechnology. We present a novel three-step method to produce nanostructures that are selectively decorated with functional molecules. We use fluorophores and nanoparticles to functionalize SiO features with defined shapes and with sizes ranging from micrometers to 25 nm. The method is called MACE-ID: molecular assembly controlled by electron-beam-induced deposition. In the first step, SiO nanostructures are written with focused electron-beam-induced deposition, a direct-writing technique. In the second step, the deposits are selectively silanized. In the final step, the silanes are functionalized with fluorescent dyes, polystyrene spheres, or gold nanoparticles. This recipe gives exciting new possibilities for combining the highly accurate control of top-down patterning (e-beam direct writing) with the rich variety of the bottom-up approach (self-assembly), leading to active or responsive surfaces. An important advantage of MACE-ID is that it can be used on substrates that already contain complex features, such as plasmonic structures, nanoantennas, and cavities.


… “samples is carried out using a CoolLED precisExcite High-power LED (465 nm) illumination attached to”…

Product Associated Features

pE-100: A range of compact, simple-to-use, single wavelength illumination systems for screening fluorescence.

Product Type



ACS Nano

Year of Publication


Country of Publication

The Netherlands