Fast moving organisms like C. elegans can be problematic for in vivo imaging – especially when trying to match up fluorescent signals with morphological information.
At the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland, Platform Head at the Facility for Advanced Imaging and Microscopy, Laurent Gelman, explains how he achieves exactly this. Thanks to a CoolLED pT-100 Illumination System for transmitted light, gene expression patterns can be automatically mapped in individual worms both spatially and temporally in vivo without movement artefacts, providing accurate insights into biological processes.
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