In fluorescence microscopy, the light hitting the sample can make all the difference between an experimental breakthrough and inconclusive results. However, when optimising an experiment, components such as the light source can often be overlooked, leading to a reduction in image quality.
In our new white paper, we explain how to achieve quality images and insightful data through improving irradiance – including which microscope components should be considered and why less is sometimes more.
This new release builds on our previous white paper (available here), where we cut through the confusion of power/intensity/irradiance and explained how to precisely measure light at the sample plane.
Read the new white paper “Optimising irradiance for fluorescence microscopy”!