Figure 1: Cas9/Ccl17-eGFP expressing bone marrow cells, transduced with a gRNA-mCherry construct, for genome editing and identification of new signaling pathways triggering allergies.
The molecular basis of allergies is not yet well understood, and this is a significant focus of research at the Hugh Green Cytometry Centre, Malaghan Institute of Medical Research in New Zealand.
In this new application note, you can find out about their work in understanding how a particular type of immune cell (T-helper type 2 cells) become activated to cause an allergic reaction.
The researchers also explain how the CoolLED pE-300ultra (won by Bioimaging Specialist Alfonso Schmidt for the Eye of Sauron Image in an Image entry!) has been a game changer for CRISPR-Cas9 screens.
Thank you to the authors for contributing this application note and choosing the charity Fair Earth Foundation to support. Find out how you can support your favourite registered charity and Share your Science!
Read the application note here
