Measuring calcium can provide many insights into all sorts of biological processes, and it is important to keep up with the latest developments as calcium imaging technology evolves. The following resources provide valuable information, whether you are new to calcium imaging or interested in exploring different applications.
An overview of Calcium Imaging
This useful guide to calcium imaging created by Dr Mark Browne and Dr Alan Mullen from Andor, explains the different fluorescent indicators developed to monitor calcium. They also explain additional experimental considerations, including the calcium imaging light source and detectors, which are especially important when working at the shorter wavelengths of ratiometric indicators such as Fura-2. Read the article here.
LEDs: the next generation calcium imaging light source
LEDs have become the go-to calcium imaging light source and the CoolLED pE-340fura is a popular LED calcium imaging light source for Fura-2 ratiometric calcium imaging, which also supports everyday fluorescence microscopy applications. It offers UV-optimised optics for FURA-2 imaging alongside the fast, controllable illumination for which CoolLED Illumination Systems are renowned.
We’re excited to share how scientists around the world use this calcium imaging light source technology, with the following interview and application notes:
At the University of Debrecen (Hungary), experts in intracellular calcium homeostasis, Professor László Csernoch, Dr Peter Szentesi and Dr Beatrix Dienes, explain their research and the importance of ratiometric calcium measurements. They recently started using the CoolLED pE-340fura Illumination System with their Zeiss Axio Vert microscope, and discuss their reasons for switching to this LED Illumination System. Read the interview here.