We recently teamed up with Scientifica, who specialises in providing cutting-edge equipment optimised for electrophysiology, multiphoton imaging and optogenetics research. In our new white paper, you can learn about a few tricks for capturing fast events with a high signal to noise ratio using the latest technologies available – and without breaking the bank!
The ability to capture fast events in detail is crucial for driving new discoveries in neuroscience research. However, as microscopy technology becomes increasingly advanced, weighing up the available options for achieving high temporal resolution within laboratory budgets can be a challenge. Moreover, LED microscopy illumination has disrupted traditional approaches to high‐speed imaging and optogenetic stimulation. In light of this (excuse the pun), we wanted to provide updated guidance to help the neuroscience community understand which filters, light sources and other components are right for each individual setup, since this can often be a source of confusion.
Read the white paper to find out:
- Which combination of widefield illumination technologies can achieve <7 μs imaging speeds
- How one filter cube is nearly always sufficient for multiplex fluorescence imaging
- What should be considered when choosing optical filters for speed and contrast
Are you attending Optogen 2022 in Paris? If you have any questions about LED illumination for neuroscience, Field Sales Manager Jo Whetstone will be there to provide more information and discuss your requirements.
Popular CoolLED Illumination Systems for neuroscience:
pE-4000 – The pE-4000 sets the standard as the universal Illumination System for fluorescence microscopy. The system has 16 selectable LED wavelengths across four channels that can be finely controlled and matched to the filters and fluorophores of almost any microscope. It is also available in a configuration to offer light delivery with a fibre, which is ideal for optogenetic stimulation.
pE-800fura – The pE-800fura offers the most comprehensive calcium imaging system available and is ideal as a go-to neuroscience illuminator. It also enhances a range of applications from pH monitoring and optogenetics to everyday fluorescence microscopy, combining lightning-fast switching with eight individually controllable channels.
pE-300ultra – The CoolLED pE-300ultra is a highly controllable fluorescence microscopy Illumination System which offers intense, broad-spectrum LED illumination for imaging most common fluorophores. Fast events can be captured with microsecond switching via multiple TTL inputs, and the ability to mount inline excitation filters allows fast, flexible filter configurations.
Many more Illumination Systems are available – please see our product comparison table for more information.