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Image Gallery

A keratinocyte undergoing mitosis in which the keratin is labelled with GFP. Acquired on a Olympus IX-83 microscope – CoolLED pE-4000. Scale bar = 10 μm.

Structured illumination superresolution microscopy (3D-SIM) images of the GFP labelled keratin within (A) a wildtype and (B) a mutant keratinocyte cell. Acquired on a GE Healthcare DeltaVision OMX Blaze microscope. Scale bar = 10 μm.

Time series of a confluent sheet of keratinocytes migrating during a live-cell imaging outgrowth assay, acquired on an Olympus IX-83 microscope equipped with a CoolLED pE-4000 lightsource (Fig. 4). Scale bar = 150 μm.

Fluorescence image of 15 zebrafish larvae (fixed and stained with acridine orange) within the field of the Mesolens.

A central portion of the image is shown enlarged at upper right:

Digitised histological section (H&E staining) of human skin acquired on a MetaSystems Slide scanner. Scale bar = 2 mm

CoolLED pE-4000 working with cultured hippocampal neurons

In these images, you can see an example of a channelrhodopsin-2 and eYFP-expressing neuron that we use for electrophysiological recording.

Image of living human oocytes showing the presence/location of the metaphase II spindle.

Images were acquired using the OosightTM system and CoolLED pE-100wht

TdTomato-expressing Aspergillus fumigatus (amber) microcolony after 12 hours development on an A549 epithelial alveolar monolayer labelled with ConA-FITC (blue)

Aspergillus fumigatus germlings loaded with a synthetic cell membrane counterstain TMR-PAF96 (amber) and treated with fluorescent BODIPYcPAF26 anti-fungal peptide (cyan) to determine peptide’s localisation and mode of entry into the pathogen.

Neurospora crassa hyphal filaments treated with fluorescently labelled siRNA (amber) to determine its localisation, with respect to nuclei (blue) and the fungal cell wall and septa (cyan).

Ultrafast endocytosis at mouse hippocampal synapses taken with a CoolLED pE-2

Antibody staining of a transcription factor localising in the nucleus

Live HeLa cells stained with lysosome marker imaged with Nanolive’s holotomographic technology.

Live FUCCI mouse embryonic stem cells imaged with Nanolive’s holotomographic technology

Fixed HeLa cells stained with Antibody for cytoskeleton (green), mitochondria (orange) and nuclear membrane(red). The whole cell was also imaged with Nanolive’s holotomographic technology and the four images were overlapped to create the overlay.

Fluorescent rat liver image taken using a CoolLED pE-300white, Olympus BX51 40x objective and a DP71 colour camera

Fluorescent kidney image taken using a CoolLED pE-300white, Olympus BX51 40x objective and a DP71 colour camera

Fluorescent bovine pulmonary artery endothelial cells image taken using a CoolLED pE-300white, Nikon 20x objective and a black and white camera

Transmitted Podcarpus Elatus Leaf image taken using a CoolLED pE-100wht, Olympus BX51 20x objective and a DP71 colour camera

Fluorescent rat liver image taken using a CoolLED pE-300white, Olympus BX51 20x objective and a DP71 colour camera

Fluorescent skin image taken using a CoolLED pE-300white, Olympus BX51 40x objective and a DP71 colour camera