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precisExcite™
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A bright, stable, long lasting light source for fluorescence microscopy
precisExcite™ is a
practical LED light source for fluorescence microscopy that delivers three
separate, controllable wavelength peaks to match the common DAPI, FITC
and Rhodamine fluorofores. It is based on a new, patent pending, high
power collimated LED light source technology.
precisExcite™ represents
the very latest in fluorescence illumination. No more throwing wide spectrums
at your sensitive samples, precisExcite™ delivers high power light in
the three wavelengths required for common fluorescence methods. Comparable
results to those produced with mercury and xenon bulb systems are achieved
but without the restrictions, limitations and on going running costs.
The three color LED sources in a single unit target all the common blue,
green and red emitting fluorophores (DAPI, GFP, FITC, CY2, CY3, Rhodamine,
Texas red etc ) and give each a precise spectrum containing extremely
low out of band noise with no deep UV or IR.
Being a solid-state triple LED light source, the output is stable over time with a long working lifetime in excess of 10,000 hours. No warm up or cool down periods are required as the light source can be switched on and off at any time to produce a consistent, pre-defined output, time after time - ideal for a multi-user facility or tissue culture fluorescence system. As the specified 10,000 hours is a total figure of LED on time, this would equate to five years life if it was used eight hours a day, every working day of the year. Even with heavy experimental usage a lifetime of 10 to 20 years can be expected.
This ultra stable source is ideal for anyone undertaking fluorescence time-course experiments or those who need to compare samples from one day, or even one year, to the next. Mercury bulbs are recommended to be left on during the working day to preserve bulb life. With its solid state LED light source, the precisExcite™ has no mercury, no arc wander that causes power fluctuations and no risks associated with exploding high pressure bulbs.
The output of each of the three wavelength sources can be precisely controlled in 1% steps, removing the need for neutral density filters that only provide a course level of adjustment. This also benefits ratio-metric tests as each wavelength source can be balanced independently prior to a test and will remain consistent throughout the experiment. The system is supplied pre-aligned for a specific microscope so there is no need for lengthy bulb image centring adjustments. Just fit the adaptor and the alignment is complete.
During the development of this product, Southampton University was used as its Beta test site facility; this is what they had to say.
"precisExcite units have replaced mercury bulb fluorescence systems in our microscopy facility with great success. A straightforward plug and play design, an easy to use control pad, adjustable intensity and fluorescence output stability has made it popular with our large user group. It is easily bright enough for all our current projects, including live cell samples, fixed tissue sections, and plant material; even penetrating deep into drosophila larvae preparations. As facility manager, I am happy that we no longer have to deal with the safety issues surrounding mercury bulbs, and even happier that I will never have to carry out bulb alignment again. We estimate that with each of the three wavelengths having 10,000 hours life expectancy, that we won't be changing our fluorescent sources for the next 15 to 25 years!"
Dr. Matt Cuttle,
Imaging Services Manager,
School of Biological Sciences,
University of Southampton.
Easy to use
- precise control of light intensity and excitation time
- controlled from either PC or remote POD
- no alignment required
- no bulb replacement
- instant on/off with no warm up/cool down time
- no more worries about the state of the mercury bulb
More control
- light intensity can be varied in 1% steps
- either manual control of the light on-time or preset time for durations from 10ms to hundreds of hours.
- stable output with no drift (<7.5% in 10,000hrs)
- fast switching between wavelengths (<10ms)
- excellent uniformity in field of view - no heat transferred to sample or microscope
Economic
- long life light source (>10,000 hours of LED on-time)
- up to 25 years of constant fluorescent illumination for typical usage.
- no bulbs to replace
- no wasted time with re-aligning new bulbs Safety and environmental issues
- built in interlocks to ensure no exposure to high intensity light (safe Class 1 LED product)
- no mercury and hence no risk of exploding high pressure bulbs
- no UVC, UVB or IR radiation
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