LED light for microscope
  • LED light for microscope
  • pE-300white Control Pod
  • LED light for microscope



CoolLED’s pE-300ultra is a fluorescence microscopy light source which offers intense, broad-spectrum LED illumination for imaging most common fluorescent stains.  Users have access to both microsecond switching via multiple TTL inputs and the ability to mount inline excitation filters.  This, when paired with today’s high performance multi band filter sets, facilitates imaging traditionally done via a white light source and a filter wheel, with all the benefits of LED.

The pE-300ultra is the most controllable member of the pE-300 Series.  In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength intensity and shuttering.  Until now these benefits have only been accessible to users of high end, highly flexible illuminators such as the CoolLED pE-4000.

Throughout 2017 we will be releasing exciting new products and product updates.  The first member of the CoolLED product range to receive the star treatment is the pE-300 Series.

As part of the pE-300 Series, the NEW Enhanced pE-300ultra  now delivers DOUBLE the intensity at the microscope sample plane.  It allows adjustment of output in 1% steps, giving precise control.  The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.



It is now possible to view and image your samples brighter than ever.

LED illumination means no mercury to dispose of and lower energy costs due to low power consumption and the precise control offered. With the new enhanced pE-300ultra, all 3 channels at full power use just 46 Watts. That is almost a quadruple efficiency increase!

This compares to other LED technology that uses 120 to 350 Watts. This leap forward in technology makes LEDs more attractive when compared to old Mercury or Metal Halide technology.  Where “green” funding is available, the reduction in institutional energy usage improves the return on investment.

The pE-300ultra  offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light.  This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.

The system comprises a pE-300ultra Light Source, Control Pod, set of three Excitation Filter Holders and Power Supply.  It can be specified with a microscope adaptor for direct fit configuration or with a 3mm diameter liquid light guide if there is a need to keep the source of illumination remote from the microscope.  An optional pE-Universal Collimator and microscope adaptor can be selected for use with a liquid light guide.

A benefit of the pE-300ultra is that  stains can be viewed either individually or in combination, without filter cube changes.  This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used.  Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.

An important additional feature of the pE-300ultra is that it includes CoolLED’s “Sequence Runner” multiple channel excitation mode.  Users can define the order of their fluorophore capture using their pE-300ultra Control Pod, then the pE-300ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels.  This feature is independent of the individual channel TTL inputs on the light source.  This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.

With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300ultra can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a safe, convenient illumination system without any additional operating costs.



There are two pE-300ultra configuration options:

Direct-fit  for connecting to a microscopes – by selecting from a range of microscope adaptors  which covers all current and most older models.  A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide with a fixed 3mm diameter, liquid light guide.  An optional pE-Universal Collimator can be specified in conjunction with a microscope adaptor if required.

For further information go to the Downloads tab

The pE-300ultra offers:-

  • Clean bright illumination across the spectrum – excites common fluorescent stains
  • Specify for  existing single and multi-band filter sets – no need to buy new filters
  • Instant on/off - No shutters required, no warm up or cool down
  • Simple to fit, simple to use – no alignment, a once only adjustment
  • Stable & repeatable – reliable and consistent results
  • Wide range of microscope adaptors – fits most microscopes
  • Precise intensity control in 1% steps (0-100%) – no ND filters required
  • Excellent uniformity over field of view – fixed and stable, no alignment necessary
  • Long lifetime – expected to exceed 25,000 hours of operating time
  • Removable inline excitation filter holders - no moving parts
  • Individual channel triggering via TTL in microseconds
  • Sequence Runner - sequenced excitation from a single TTL-out

DAPI can be excited at either 365nm or 400nm and microscopes typically have a filter set which allows one or other of these wavelengths to be transmitted.  To accommodate this, the pE-300ultra can be specified in one of two waveband configurations to match with the user’s existing filter sets within the microscope.

A microscope which is populated with a number of single band (“SB”) filter sets will typically have DAPI excitation at 365nm and a microscope with multi-band (“MB”) filter(s) has excitation at 400nm.  The user can specify the configuration which is appropriate for their microscope.

Power requirements
100-240V a.c. 50/60Hz, 1.4A

Power consumption
Standby Max 2W
1 band (GYR) at 100% intensity Max 20W
2 bands (BLU & GYR) at 100% intensity Max 38W
White (all 3 bands) at 100% intensity 46W


pE-300ultra Light Source: 77mm(w) x 186mm(d) x 162mm(h) - Weight 1.4kg
pE-300ultra Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg
pE-300ultra Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg

pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg

Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

  • Mercury-free
  • Energy Efficient: 80% less power
  • Long lifetime (25,000 operating hours)
  • No bulb replacements
  • Reduced risk of eye damage
  • Quiet operation
  • No special disposal regulations or issues

System = 36 months, LEDs = 36 months

Control pod for operation and access to settings and usage history.

Manual control for instant on/off and intensity control in 1% steps from 0 – 100%
Individual bands can be selected/deselected and controlled independently as desired

Via global and individual channel TTL for on/off control of selected channels using a BNC connection on the light source.
Triggering speed <20us at full power

Remote via USB (B type) for imaging software control (see Imaging Software)

Sequence Runner:
Single TTL input to step through user defined sequence. 
Triggering speed <20us at full power

Direct-fit to a microscope
Specify from CoolLED’s range of epi-fluorescent or transmitted port microscope adaptors

Liquid Light-Guide
1.5m long, 3mm diameter light-guide.

pE-300-UT-D-SB-YYY-ZZ: pE-300ultra direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, set of three excitation filter holders (25mm dia.) and power supply. Single band filter set configuration
pE-300-UT-D-MB-YYY-ZZ: pE-300ultra direct fit Light Source, exchangeable microscope adaptor to customer-specified microscope, remote manual control pod, set of three excitation filter holders (25mm dia.) and power supply. Multi band filter set configuration
pE-300-UT-L-SB-ZZ: pE-300white Light Source for use with 3mm Liquid Light Guide.  Single Band filter configuration.  Includes light source, control pod, set of three excitation filter holders (25mm dia.) and power supply
pE-300-UT-L-MB-ZZ: pE-300white Illumination System - Multi-Band.  For use with 3mm Liquid Light Guide.  Multi band filter configuration.  Includes light source, control pod, set of three excitation filter holders (25mm dia.) and power supply
pE-1906 1.5m long, 3mm diameter liquid light guide
pE-10400-YYY pE-Universal collimator for use with a single liquid light guide. Includes Microscope Adaptor to customer-specified microscope

To specify microscope code (YYY) see Adaptors
To specify power cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA

CoolLED pE-300 Series Datasheet
CoolLED pE-300ultra Optogenetics Leaflet
CoolLED pE-300white / pE-300ultra Improved Multi-stain Testing

CoolLED pE-300 Series Declaration of Conformity 

pE-10400-YYY:   pE-Universal Collimator for use with a single liquid light guide. Includes customer specified microscope adaptor


To specify microscope code (YYY) see Adaptors


The pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.

The table below is a quick reference guide to the features offered by the pE-300 Series systems

For more detailed information, please visit the product page by clicking the relevant link below.