Fluorescence Microscopy


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precisExcite

PrecisExciteŽ represents the very latest in fluorescence illumination. It is a practical LED light source for fluorescence microscopy that delivers separate, controllable wavelength peaks to match the common DAPI, FITC and Rhodamine fluorophores. It is based on a new, patent pending, high power collimated LED light source technology.

Comparable results to those produced with mercury and xenon bulb systems are achieved but without the restrictions, limitations and on going running costs. The three colour LED sources in a standard unit target all the common blue, green and red emitting fluorophores (DAPI, GFP, FITC, CY2, CY3, Rhodamine, Texas Red, etc. ) providing a precise spectrum containing extremely low out-of-band noise with no deep UV or IR.

Being a solid-state triple LED light source, the output is extremely stable and has a working lifetime in excess of 10,000 hours. No warm up or cool down periods are required as the light source can be switched on and off at any time to produce a consistent, pre-defined output, time after time - ideal for a multi-user facility or tissue culture fluorescence system. With a specified 10,000 hours lifetime, the user can expect the precisExciteŽ to operate in excess of 10 years, even under regular use conditions.

This LED source is ideal for anyone undertaking fluorescence time-course experiments or those who regularly need to compare samples from one day, or even one year, to the next. Mercury bulbs are recommended to be left on during the working day to preserve bulb life. With its solid state LED light source, the precisExciteŽ has no mercury, no arc wander that causes power fluctuations and no risks associated with exploding high pressure bulbs.

The output of each of the three wavelength sources can be precisely controlled in 1% steps, removing the need for neutral density filters that only provide a course level of adjustment. This also benefits ratio-metric tests as each wavelength source can be balanced independently prior to a test and will remain consistent throughout the experiment. The system is supplied pre-aligned for a specific microscope so there is no need for lengthy bulb image centering adjustments. Just fit the adaptor and the alignment is complete.

 

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