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home I what is...? I precisExcite I application notes I distributors I news I benefits I endorsements I filters I gallery I contact precisExcite BenefitsSimply Better Control from precisExciteŽ fluorescence excitation system
Light Sources In order to generate enough excitation light intensity to furnish secondary fluorescence emission capable of detection, powerful light sources were needed. These were usually either mercury or xenon arc (burner) lamps, which produce high-intensity illumination powerful enough to image faintly visible fluorescence specimens. With the introduction of precisExciteŽ this has now all changed, where instead of high intensity arc lamps the illumination is now provided by high intensity LED /Light Emitting Diodes supplying light fluxes of similar intensities without the any of the drawbacks of conventional arc systems. Focusing and Alignment of Arc Lamps Mercury and xenon arc lamps are now widely utilized as illumination sources for a large number of investigations in widefield fluorescence microscopy. These lamps all require aligning and focusing the arc lamp in a Mercury or Xenon Burner with the Microscope, but with the advent of precisExciteŽ this has now been made a thing of the past as zero alignment is required for the 10,000 hour lifetime of the LED source. Fluorescence Photomicrography Photomicrography under fluorescence illumination conditions presents a unique set of circumstances posing special problems for the microscopist. Exposure times are often exceedingly long, the specimen's fluorescence may fade during exposure, and totally black backgrounds often inadvertently signal light meters to suggest overexposure. Added to this, existing bulb technology further complicates the results achieved as the light output of the bulb degrades very quickly and therefore the microscopist has to determine whether it is bulb degradation or the specimen's fluorescence degradation that he is measuring. The precisExciteŽ changes all of this as the light output degradation is measured over 1000's of hours of continuous use and not 10's of hours of use. Using this light source, microscopists will be better able to characterize their results as they will not have to correct for bulb intensity degradation in their findings.
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