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Application Notes
In order to assist fluorescence microscopists who are using, or will use, the precisExciteŽ high-power LED excitation source, CoolLED is compiling a database of Application Notes outlining the activities and success of the unit in relation to specific studies.
We welcome further contributions and hope that this section on of our website proves useful to all visitors. If you wish to provide an Application Note, please contact us.
| Application Note 1 | |
"Living Cells" |
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Summary:
Experiences of using precisExcite with live cell imaging |
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The following time-lapse movies should be viewed in conjunction with Application Note 1. They both show epithelial cells derived from the lining of the human airway.
Video 1 shows cells being adversely affected (note cell blebbing, cell round up and then stop moving) due to overexposure to mercury lamp emissions.
| Watch Video 1 in Windows format |  |
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Watch Video 1 in realPlayer format |  |
Video 2 shows
similar cells with fluorescently stained nuclei showing no detrimental
effects using the precisExciteŽ LED source.
| Watch Video 2 in Windows format | |
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Watch Video 2 in realPlayer format | |
| Application Note 2 | |
"Does precisExciteŽ have the power?" |
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Summary:
One persons experience of the benefits of stability over intensity
using precisExcite |
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