LEDs for Life Sciences

Why LEDs?
Why LEDs for Life Sciences?
Why CoolLED LEDs for Life Sciences?

Why LEDs?

LEDs (Light Emitting Diodes) are semiconductor devices which emit photons around a specific peak wavelength when an electrical current is passed through them.  As semiconductors, they are:

  • Fast switching
  • Reliable
  • Stable
  • Long lasting

 

single LED die excitation

 

 

 

 

High light intensities are achievable. Although LEDs are more than three times as efficient as conventional incandescent bulbs, they are only  20 – 30% efficient.  High brightness results in high heat flux densities so it is essential to provide the appropriate cooling to ensure that the LEDs do not overheat resulting in spectral drift and premature failure.

Why LEDs for Life Sciences?

The conventional light source for fluorescence is a high pressure mercury or metal halide bulb which creates multiple peaks across a broad spectrum of energy. The majority of this energy needs to be removed by filtering in order to create the excitation band required.

Hg excitation curves 

 

 

 

 

 

 

 

 

The benefits of LEDs can be considered from three aspects


Operational                                           Experimental                                                Environmental

Instant on/off                                         Fast switching- no shutters                       No mercury
No warm up time                                 Simple intensity control
No cool down time                              Simple systems integration                       No risk of exploding bulbs
No bulb replacement                          Stable output for many years  
No bulb alignment                               Reduced photobleaching                          Low energy usage

 

Why CoolLED LEDs for Life Sciences?

Only CoolLED is using active-cooling of LEDs in their excitation products.  As discussed above, LEDs are more efficient than conventional light sources but still produce much heat.  With CoolLED LED products, this heat is removed by active-cooling so that the LEDs are held within a constant temperature range.  By controlling the temperature, CoolLED can drive the LEDs more powerfully.   This results in greater intensities.  In addition, by active-cooling, the stability, reliability, and lifetime of the LEDs is greater.

It is CoolLED's expertise in LED assembly and the use of active-cooling which means that it can offer intensities up to 5x greater than other LED excitation products using commercially available packaged LED parts.

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