Optical Filters

CoolLED recommends that excitation filters are used with its LED excitation products.  Although LEDs produce a narrowband of excitation, there can be a small "tail" of excitation to shorter and longer wavelengths which may be undesirable for some applications.

Typically, if you have existing filters, these can be used with your CoolLED system.  We are happy to discuss this with you and provide our recommendations to assist you to achieve the best LED wavelength - filter combination.

The tables below provide information on commercially available filters - and filter sets - matched to CoolLED's range of LED excitation wavelengths. These are regularly updated as new LED wavelengths become available.  By clicking on a selected filter, the link will take you through to the suppliers website where you can view spectral details of the filter.

Single Band Filter sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
365nm DAPI, Hoechst 11000v3
49000
XF05-2
XF06
DAPI-5060B
380nm DAPI, Hoechst
CFW
  XF131
XF10-2
XF129-2
DAPI-1160A
DAPI-5060B
CFW-LP01-Clinical
CFW-BP01-Clinical
BFP-A-Basic
400nm DAPI 11005v2 XF106-2
XF11
DAPI-5060B
BFP-A-Basic
425nm CFP 11003v2 XF14-2 CFP-2432A
CFP-A-Basic
440nm CFP 49001 XF18-2
XF114-2
CFP-2432A
CFP-A-Basic
470nm GFP, FITC, Alexa488 11001v2
49002
XF100-2
XF100-3
GFP-3035B
FITC-3540B
GFP-A-Basic
FITC-LP01-Clinical
FITC-A-Basic
490nm FITC, GFP 49012 XF144 FITC-3540B
FITC-A-Basic
YFP-A-Basic
500nm YFP 49003 XF104-2 YFP-2427A
YFP-A-Basic
525nm Cy3 11007v2 XF101-2 CY3-4040B
535nm Cy3, Rhodamine 11007v2
49004
XF101-2 TRITC-A
CY3-4040B
TRITC-A-Basic
550nm Cy3, TRITC, DsRed,
mCherry, Texas Red
49004
49005
49008
XF40-2
XF146
TRITC-A
CY3-4040B
TXRED-4040B
TRITC-A-Basic
TxRed-A-Basic
565nm mCherry, Texas Red 11010v2
49008
  TXRED-4040B
mCherry-A
Cy3.5-A-Basic
TRITC-A-Basic
585nm APC, mCherry 31006   mCherry-A
Cy3.5-A-Basic
595nm APC 31006    
615nm        
635nm Cy5 49006 XF147
XF110-2
CY5-4040A
CY5.5-A
700nm Cy7 49007   CY7-A
770nm ICG     ICG-A

  

Multiband Filter sets

  1. Full multiband sets. Three piece set consisting of dual band excitation, emission and dichroic mirror.
  2. Pinkel sets. Four piece set consisting of 2 separate single band excitation filters and single dual band emission filter and dichroic mirror.
  3. Sedat sets. Five piece sets consisting of 2 separate single bandpass excitation filters, 2 separate single bandpass emission filters and a single dual band dichroic mirror.
Multibandpass sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
  DAPI/FITC
or
DAPI/GFP
    DA/FI-A
380 & 470nm or
380 & 490nm
  DAPI/TRITC      
  CFP/YFP 59017
425nm or 440nm &
490nm or 500nm
  CFP/YFP-A
425 & 500nm
  GFP & DsRed
or TRITC
    GFP/DsRed-A
470nm & 550nm
  FITC/Texas Red or
GFP/mCherry
59022
470nm or 490nm &
565nm or 585nm
  FITC/TxRed-A
470nm & 585nm or
490nm & 585nm
  Cy3/Cy5     Cy3/Cy5-A
535nm & 635nm
  DAPI
FITC
TRITC
69000
400nm & 470nm or
490nm & 550nm
  DA/FI/TR-A
380nm & 470nm
& 550nm
  DAPI
FITC
Texas Red
69002
400nm &490nm &
565nm or 585nm
  DA/FI/TX-B
400nm & 490nm &
565nm or 585nm


Pinkel sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
  DAPI/FITC
or
DAPI/GFP
    DA/FI-2X-A
380nm & 490nm
  DAPI/TRITC      
  CFP/YFP 59217
425nm or 440nm
& 500nm
  CFP/YFP-2X-A
425nm & 500nm
  GFP &
DsRed or TRITC
    GFP/DsRed-SX-A
470nm & 550nm
  FITC/Texas Red or
GFP/mCherry
59222
470nm & 565nm
or 585nm
  FITC/TXRED-2X-A
470nm & 585nm
GFP/HcRed-2X-A
470nm & 585nm
  Cy3/Cy5     CY3/CY5-2X-B
535nm or
550nm & 635nm
  DAPI
FITC or GFP
TRITC or Cy3
62000v3
365nm & 470nm
or 490nm & 550nm
  DA/FI/TR-3X-A
380nm & 470nm
& 550nm
  DAPI
FITC or GFP
Texas Red
69302
400nm & 490nm &
565nm or 585nm
  BFP/GFP/HcRed-3X-A
365nm or 380nm &
470nm & 585nm
DA/FI/TX-3X-A
380nm & 490nm &
565nm or 585nm
  CFP
YFP
TxRed or mCherry
69308
425nm or 440nm &
500nm & 565nm or 585nm
  CFP/YFP/HcRed-3X-A
425nm & 500nm
& 585nm


Sedat sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
  DAPI/FITC
or
DAPI/GFP
    DA/FI-2X2M-A
380nm & 490nm
  DAPI/TRITC      
  CFP/YFP 89002
425nm or 440nm &
490nm or 500nm
  CFP/YFP-2X2M-A
425nm & 500nm
  GFP &
DsRed or TRITC
89019
470nm or 490nm &
550nmm
  GFP/DsRed-2X2M-B
470nm & 550nm
  FITC/Texas Red &
Red or mCherry
89017
490nm & 565nm
or 585nm
89021
470nm & 565nm
or 585nm
  FITC/TXRED-2X2M-A
490nm & 585nm
or 595nm
  Cy3/Cy5     CY3/CY5-2X2M-B
525nm or 535nm
or 550nm & 635nm
  DAPI
FITC
TRITC
    DA/FI/TR-3X3M-A
380nm & 470nm or
490nm & 550nm
  DAPI
FITC
Texas Red
89014
400nm & 490nm &
565nm or 585nm
  DA/FI/TX-3X3M-A
380nm & 490nm &
565nm or 585nm
  CFP
YFP
mCherry
89006
440nm & 490nm or
500nm & 565nm or 585nm
  CFP/YFP/HcRed-3X3M-A
425nm & 500nm
& 585nm


FRET sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
  BFP/GFP     FRET-BFP/GFP-A
380nm
  CFP/YFP 49052
440nm
  FRET-CFP/YFP-A
440nm
  GFP/RFP   XF151-2
470nm
FRET-GFP/RFP-A
470nm


FISH sets
LED
peak λ
Common
Fluorophore
Chroma Omega Semrock
  DAPI     DAPI-1160A
380nm
  SpectrumAqua     SpAqua-A
425nm or 440nm
  SpecrtumGreen     SpGr-A
490nm
  SpectrumGold     SpGold-A
525nm or 535nm
  SpectrumOrange     SpOr-A
535nm or 550nm
  SpectrumRed     SpRed-A
585nm
  Cy5
SpectrumFRed
    CY5-4040A
635nm
  Cy5.5     CY5.5-A
635nm
  Cy7     CY7-A
700nm
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