Users' Comments

A selection of User's comments are listed below.  If you would like to contact an existing user to discuss the suitability of a CoolLED LED product for your application, contact CoolLED for further details.

"We have recently established a visualisation process for small GTPase activity using FRET.  We used a CoolLED pE-2 LED system to excite the fluorophores.  It proved to be an essential tool in the acquisition and processing of the FRET images by (inter alia) its ability to minimise photobleaching".
   Kazuhiro Aoki & Michiyuki Matsuda, School of Biostudies, Kyoto University

 "Following comparative testing, we selected CoolLED's LED systems.  Intensity, ease of control, and integration with our selected imaging software, were the reason for making this selection.  We now have 12 CoolLED pE-1 and pE-2 units and plan more in the future.  We like CoolLED's flexible approach and found the company to be responsive and supportive".
      Pawel Pasierbek, IMP, Vienna

"I work on experimental epifluorescence systems using a novel objective lens of low magnification and high N.A.  I have found the CoolLED pE-1 illumination system extremely useful and convenient, particularly because I need precisely constant power over both milliseconds and hours, and I can work much faster if there is no warm-up problem.  I have also found the company very helpful".
      Brad Amos. LMB, Cambridge

"We now have a number of CoolLED LED systems.  The most recent has been fitted to a live cell imaging system which was rarely used.  Now that users have realised how stable the light source is and how easy it is to control a CoolLED system, the microscope is used almost full time.  This is a great use of lab resources where a small investment in a light source has renewed a microscope system".
      Peter March, Faculty of Life Sciences, Manchester University

" We have had great success using the CoolLED system for high speed imaging of fluorescent protein pairs ECFP/EYFP and EGFP/dsRed. We get consistent results at speeds not possible with bulb and shutter/ filter wheel systems".
      Jens Rietdorf, Friedrich-Miescher Institut / Novartis, Basel

"We have a CoolLED LED unit on a simple tissue culture microscope that is used by many people.  The unit is perfect in this situation because it is aligned only once, very simple to use, instantly on or off, controllable, doesn't require a PC to control it, doesn't get hot and there's no bulb to fail at an inconvenient time.  It won't take up any of my time in the future - which is perfect!  I can happily leave the unit in a tissue culture suite knowing that there are no safety or maintenance issues".
      Paul Appleton, Cell & Developmental Biology, Dundee University

"We perform quantitative ratio imaging in living cells. The most appealing performance characteristics of the CoolLED LED excitation system for us are: very good stability of light intensity, essential for quantitative work - no arc wandering, fluttering or flaring issues, fast vibration free switching of wavelengths, suitable for live cell imaging (no unintentional UV exposure), easy adjustment of light intensity to reduce photo bleaching and photo toxicity, and controllable via imaging software packages".
      Harri Mustonen, Department of Surgery, Helsinki University Hospital

"The main benefit of CoolLED's light source is the ability to switch on and off when required and also not having to align or change a bulb every 300 hours. Over the years it will pay for itself when compared to the cost of mercury bulbs".
      Kevin MacKenzie, Histology/EM and Imaging Facility, Institute of Medical Sciences, Aberdeen University