Research (3)

Many Research applications use Live Cell Imaging

Live Cell Imaging is an important and growing area of fluorescence microscopy.  It is preferable to examine proteins in living cells themselves as this offers insights not possible using fixed cell techniques.

Today's live cell imaging systems can offer the resolution required to image living cells without destroying them.  In addition, developments in fluorescent dyes and proteins have further facilitated the study of complex cellular processes using fluorescent staining (“labeling”) of various proteins, ions and lipids in living cells. 

However, the types and duration of cellular processes that can be imaged have been constrained in microscopy systems by limitations in acquisition speed (camera), mechanical delay (shutters, filters), and by phototoxicity and photobleaching (light sources).   Cameras are now available which offer high acquisition speeds.  The remaining problems are addressed using a CoolLED LED excitation system. 

With a CoolLED LED system for Live Cell Imaging, you have....

• Microsecond switching under TTL reduces delays in image capture sequences
• Reduced UV from LEDs means less phototoxicity
• No Shutters & ND Filters means less vibration
• No Shutters means no mechanical delay in the image capture sequence
• Remote source means no heat or electro-magnetic interference near samples

Typical Live Cell configurations from CoolLED use the pE-2 excitation system.  

See specific pages on Time Lapse, Dual Staining, and Improved Cell Viability.